Fungalpedia – Note 8 Anisofilariata
Anisofilariata Y.S. Tokarev, V.N. Voronin, E.V. Seliverstova, V.V. Dolgikh, O.A. Pavlova, A.N. Ignatieva & I.V. Issi
Citation if using this entry: Fahalli et al. (2023) New genera in 2010-2011. Mycosphere (in press)
The monotypic genus Anisofilariata was introduced by Tokarev et al (2010) with an uncertain position in Microsporidia, Protozoa with Anisofilariata chironomi as the type species. The strain was isolated from larvae of Chironomus plumosus as a parasite, in north-western Russia (Tokarev et al. 2010). The genus name refers to the anisofilar polar filament, one of the distinguishing features of the genus. This microsporidium has broad, oval, uninucleate spores in sporophorous vesicles, and is distinct from other known microsporidian species by sporogony and spore ultrastructure that suggests the establishment of a new genus and species.
Fig 1 –Anisofilariata chironomid (Photo credits: Collection of Prof. Irma V. Issi, provided by Yuri Tokarev, All-Russian Institute of Plant Protection, St. Petersburg). a A spore with anchoring disc (Ad), polar filament (Pt), nucleus (N), anterior (Pp1), and posterior parts (Pp2) of the polaroplast. b A sporophorous vesicle containing three spores. c Spores on Giemsa-stained smears. d Fresh spores of A. chironomi. Scale bars: a-b=10 μm, c-d=2.5 μm.
The spore measurements vary depending on the number of spores in the sporophorous vesicle. The polaroplast has two anterior and posterior parts composed of very thin and thick lamellae and occupies most of the spore. The sporophorous vesicle is enclosed by a thin membrane and contains several tubular structures. Phylogenetic analysis based on SSU showed no close phylogenetic relationships between Anisofilariata chironomi and microsporidia from other dipteran hosts (Tokarev et al. 2010).
Type species: Anisofilariata chironomi Y.S. Tokarev, V.N. Voronin, E.V. Seliverstova, V.V. Dolgikh, O.A. Pavlova, A.N. Ignatieva & I.V. Issi
Tokarev Y S, Voronin VN, Seliverstova EV, Dolgikh VV, Pavlova OA, Ignatieva AN, Issi IV. 2010 – Ultrastructure and molecular phylogeny of Anisofilariata chironomi gn sp. n. (Microsporidia: Terresporidia) from Chironomus plumosus L. (Diptera: Chironomidae). Parasitology research 107, 39-46. https://link.springer.com/article/10.1007/s00436-010-1830-1
Maryam Fallahi, Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand.
(Edited by Kevin D Hyde)