Fungalpedia – Note 704, Zeloasperisporium
Zeloasperisporium R.F. Castañeda.
Citation when using this data: D. S. Marasinghe et al. 2023 (in prep.) – Fungalpedia, Ascomycota.
Index Fungorum, Facesoffungi, MycoBank, GenBank., Figs 1,2,3
Classification: Zeloasperisporiaceae, Zeloasperisporiales, Pleosporomycetidae, Dothideomycetes, Pezizomycotina, Ascomycota, Fungi
Epiphytic on the surface of living leaves, stems or branches appearing as blackened spots. Sexual morph: Thyriothecia solitary and scattered, orbicular, superficial, brown to dark brown, ostiole absent. Upper wall composed of ellipsoid to angular cells, arranged in parallel radiating lines from the centre to the outer rim with darkened cells. Hamathecium lacking pseudoparaphyses. Asci 8-spored, bitunicate, fissitunicate, globose to ovoid or clavate, apedicellate, with apical ocular chamber. Ascospores 2–3-seriate, obovoid to clavate, hyaline, 1-septum, widest in upper cell, smooth-walled. Asexual morph: Mycelium superficial to immersed, pale brown or olivaceous to brown, septate, smooth or finely verrucose, thin-walled. Macroconidiophores arising as lateral hyphal branches, erect, straight, subcylindrical or conical, tapering towards the apex, medium-brown, minutely verrucose; conidial proliferation sympodial, with one to several subdenticulate to flat conidiogenous loci, mostly crowded at the apex, protuberant; conidial scars thickened-refractive. Macroconidia solitary, straight to curved, fusiform or cylindrical or obclavate or clavate, tapered towards the apex, septate, olivaceous to pale to medium brown, verrucose; at times forming a globose, apical mucoid appendage; base truncate with a protruding scar, which is somewhat thick-ened and darkened-refractive. Micronematous synanamorph. Conidiogenous cells short-cylindrical, brown, finely verrucose, as lateral pegs on hyphae, with minute apical scars. Microconidia obovoid, aseptate, brown, finely verrucose (Castañeda Ruiz et al. 1996; Crous et al. 2007; Hongsanan et al. 2015d).
Notes: Castañeda Ruiz et al. (1996) introduced Zeloasperisporium to accommodate the asexual morph of Zeloasperisporium hyphopodioides which was isolated from air in Cuba and was not accommodated in any family. Crous et al. (2007) placed this genus in Venturiaceae based on sequence data of the type species. Later, Crous et al. (2015) established Zeloasperisporiaceae to accommodate Neomicrothyrium and Zeloasperisporium (Hongsanan et al. 2015d; Jayasiri et al. 2018). Hongsanan et al. (2015d) synonymized Neomicrothyrium under Zeloasperisporium based on morphology and established Zeloasperisporiales. Species of this order are characterized by circular, flattened, brown to dark brown thyriothecia lacking ostiole, globose to ovoid or clavate, apedicellate asci with obovoid to clavate, 1-septum ascospores and obovoid, aseptate, brown microconidia (Hongsanan et al. 2015d). According to our phylogenetic analysis, Zeloasperisporium formed an independent lineage between Phaeotrichales and Natipusillales with 100% ML BS, 1.00 BY PP support
Type species Zeloasperisporium hyphopodioides R.F. Castañeda, in Castañeda Ruíz et al., Mycotaxon 60: 285 (1996).
Other accepted species: species Fungorum – search Zeloasperisporium.
Figures 1 – Zeloasperisporium hyphopodioides. a Conidi- ophores. b Conidia. c Conidio- phores with conidia. Scale bars: a–c = 10 µm. Redrawn from Castañeda Ruiz et al. (1996)
Figures 2 – Zeloasperisporium sp. (Material examined: Thailand,Chiang Rai, Tasud, on stem of Allamanda cathartica L. (Apocynaceae), 09 October 2020, Diana Sandamali). a Dead stem. b Thyriothecium on the the stem. c Squash mount of thyriothecium. d Section through thyriothecium. e Upper wall of thyriothecium. f–i Globose asci with 8 ascospores. j, k Ascospores. Scale bars: c, e = 50 µm, d, f = 20 µm, g–j = 10 µm, k–m = 5 µm
Figures 3 – Phylogenetic tree generated from maximum likelihood analy- sis based on LSU and ITS sequence data for 273 strains representing epifoliar fungal species in Dothideomycetes, Eurotiomycetes, Lecano- romycetes and Sordariomycetes. Armillaria mellea (GLM 45831) was used as the outgroup taxon. The tree topology of the maximum-like-lihood analysis is similar to the Bayesian analysis. Bootstrap support values for maximum likelihood (ML) equal to or greater than 90% are given above or below the nodes. Branches with Bayesian poste- rior probabilities (BPP) equal to or higher than 0.90 are given above or below the nodes. The hyphen (“–”) indicates a value lower than 90% for ML BS or 0.90 for BY PP. Ex-type, holotype and ex-epitype strains are in bold. Epifoliar taxa of each clades are in red
Figures 3 – Continued
Figures 3 – Continued
Figures 3 – Continued
References
Entry by
Diana Sandamali Marasinghe, Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand, Mushroom Research Foundation, 128 M.3 Ban Pa Deng T. Pa Pae, A. Mae Taeng, Chiang Mai, 50150, Thailand
Published online 29 August 2023