Fungalpedia – Note 1010, Cyphellophora

 

Cyphellophora. G.A. de Vries.

Citation when using this data: Tian Q et al. 2021 (in prep.) – Fungalpedia, Ascomycota.

Index Fungorum, Facesoffungi, MycoBank, GenBank., Fig 1

Classification: Cyphellophoraceae, Chaetothyriales, Chaetothyriomycetidae, Eurotiomycetes, Pezizomycotina, Ascomycota, Fungi

Epiphytic, saprobic and pathogenic on a range of hosts worldwide. Colonies mostly growing slowly, pale grey-brown in the center, loose, cottony, woolly-velvety, margin entire, flat, straight or sharp, dark brown to olivaceous black, reverse olivaceous black, somewhat moist. Vegetative hyphae hyaline initially, pale brown in older hyphae, septate, constrictions at the septa, straight or undulate, smooth-walled, guttulate or aguttulate, with or without oil droplets. Sexual morph: Ascomata scattered, subglobose to globose, dark brown, glabrous, thick-walled, ostiolate or ostiole inconspicuous, with or without dark superficial hyphae. Wall of ascoma multi-layered, comprising brown to hyaline cells of textura angularis and textura globulosa. Asci 8-spored, bitunicate, ellipsoidal to cylindrical, ovoid to ampulliform, with a short pedicel. Ascospores 2–3-seriate, ellipsoidal to fusiform, hyaline, 1–3 septate, not constricted at the septa, narrowly rounded at the ends, with or without a guttule in each cell (Yang et al. 2018, Phookamsak et al. 2019). Asexual morph: Hyphomyctous. Conidiophores absent or rarely reduced to a short cell, basal to the conidiogenous cells. Conidiogenous cells enteroblastic, phialidic. short cylindrical to flask-shaped, intercalary, lateral or terminal, sometimes arising at short side branches of hyphae, with indistinct sessile collarettes or short and flaring to funnel-shaped collarettes, sub-hyaline to pale olivaceous brown, producing subsequent conidia in more or less sympodial order, thin-walled. Conidia aggregated, oblong-fusoid or oblong-ovoid, 1–multi-septate, constrictioned at the septa, hyaline to brown, straight or curved, smooth-walled, guttulate or aguttulate, with or without oil droplets, without a gelatinous sheath or appendages. Spermatial state absent. Chlamydospores absent (de Vries 1962, Feng et al. 2014).

Notes – Cyphellophora G.A. de Vries is widespread and is ecologically important. Species of Cyphellophora comprise; (1) mostly human and animal pathogens, such as
C. laciniata G.A. de Vries (type species), C. europaea (de Hoog, Mayser & Haase) Réblová & Unter. and C. pluriseptata G.A. de Vries which were isolated from nails or skin of humans
(de Vries 1962, de Hoog et al. 2000a, Feng et al. 2012, 2014), C. phyllostachysdis G.Y.
Sun & Liu Gao, C. europaea, from a human or mammal eyes, resulting in infection of hyperkeratosis (de Hoog et al. 2000a); (2) saprobes, such as C. filici isolated from dead fronds of a fern, without superficial dark hyphae (Phookamsak et al. 2019), C. oxyspora (W. Gams) Réblová & Unter. isolated from a decaying leaf of Clerodendron monahassa (Vu et al. 2019); (3) epiphytes, such as C. jingdongensis which can reduce photosynthesis, but does not cause plant disease (Chomnunti et al. 2014Yang et al. 2018), and (4) plant pathogens, such as
C. phyllostachysdis G.Y. Sun & Liu Gao and C. sessilis (de Hoog) Réblová & Unter. causing sooty blotch and flyspeck disease of bamboo, resulting in economic damage (de Hoog et al. 2000, Decock et al. 2003, Zhuang et al. 2010, Gleason et al. 2011, Gao et al. 2015). 

To date, 26 species are accepted in Cyphellophora, with two sexual morph species,
C. jingdongensis isolated from living leaves of Alnus nepalensis (Yang et al. 2018) and C. filici isolated on dead fronds of a fern (Phookamsak et al. 2019). There are nine species reported from plant material, namely as C. artocarpi G.Y. Sun & Liu Gao, C. filici, C. guyanensis Decock & G. Delgado, C. jingdongensis, C. musae, C. olivacea (W. Gams) Réblová & Unter., C. oxyspora,
C. phyllostachydis and C. sessilis (Gams & Holubová-Jechová 1976, de Hoog et al. 1999, Decock et al. 2003, Gao et al. 2015, Yang et al. 2018, Phookamsak et al. 2019). 

The asexual morphs of Cyphellophora are recognized as black yeasts which are difficult to identify solely based on morphology. Cyphellophora resembles black yeasts, such as Phialophora Medlar and Pseudomicrodochium B. Sutton, but they differ from each other in having different conidia and thallus colours (Decock et al. 2003, de Hoog et al. 2011, Réblová et al. 2013). Thus, a combination of morphology, ecological traits and phylogenetic analyses provide accurate generic and species delimitation in Cyphellophora. Phylogenetic studies have shown that Cyphellophora clustered in a well-supported clade within Chaetothyriales (Feng et al. 2014, Gao et al. 2015, Yang et al. 2018, Phookamsak et al. 2019).

Type species: Cyphellophora laciniata G.A. de Vries.

Other accepted species: Species Fungorum – search Cyphellophora.

 

image

 

Figure 1 – Cyphellophora laciniata (Centraalbureau voor Schimmelcultures, holotype, redrawn from de Vries 1962). a–e Conidiogenous cells with conidia. f Conidia. Scale bar: 20 μm.

 

References

Chomnunti P, Hongsanan S, Aguirre-hudson B, Tian Q et al. 2014 – The sooty moulds. Fungal Diversity 66, 1–36.

de Hoog GS, Guarro J, Gené J, Figueras MJ. 2000a – Atlas of Clinical Fungi, 2nd ed. Centraalbureau voor Schimmelcultures, Utrecht, Universitat Rovira i Virgili, Reus.

de Hoog GS, Vicente VA, Najafzadeh M, Harrak M et al. 2011 – Waterborne Exophiala species causing disease in cold-blooded animals. Persoonia 27, 46–72.

de Hoog GS, Zalar P, Urzi C, de Leo F et al. 1999 – Relationships of dothideaceous black yeasts and meristematic fungi based on 5.8S and ITS2 rDNA sequence comparison. Studies in Mycology 43, 31–37.

de Vries GA. 1962 – Cyphellophora laciniata nov. gen., nov. sp., and Dactylium fusarioides Fragoso et Ciferri. Mycopathologia et mycologia applicata 16, 47–54.

Decock C, Celgado-Rodriguez G, Buchet S, Seng JM. 2003 – A new species and three new combination in Cyphellophora, with a note on the taxonomic affinities of the genus, and its relation to Kumbhamaya and Pseudomicrodochium. Antonie van Leewenhoek 84, 209–216.

Feng P, Lu Q, Najafzadeh MJ, van den Ende AG et al. 2012 – Cyphellophora and its relatives in Phialophora: biodiversity and possible role in human infection. Fungal Diversity 65, 17–45.

Feng P, Lu Q, Najafzadeh MJ, van den Ende AG et al. 2014 – Cyphellophora and its relatives in Phialophora: biodiversity and possible role in human infection. Fungal Diversity 65, 17–45.

Gams W, Holubová-Jechová V. 1976 – Chloridium and some other dematiaceous hyphomycetes growing on decaying wood. Studies in Mycology 13, 1–99.

Gao L, Ma Y, Zhao W, Wei Z et al. 2015 – Three New Species of Cyphellophora (Chaetothyriales) Associated with Sooty Blotch and Flyspeck. PLoS One 10, e0136857.

Gleason ML, Batzer JC, Sun GY, Zhang R et al. 2011 – A new view of sooty blotch and flyspeck. Plant Disease 95, 368–383.

Phookamsak R, Hyde KD, Jeewon R, Bhat DJ et al. 2019 – Fungal Diversityity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal diversity 95(1), 1–273.

Réblová M, Untereiner WA, Réblová K. 2013 – Novel evolutionary lineages revealed in the Chaetothyriales (Fungi) based on multigene phylogenetic analyses and comparison of ITS secondary structure. PLoS ONE 8(5), e63547.

Vu D, Groenewald M, de Vries M, Gehrmann T et al. 2019 – Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92, 135–154.

Yang H, Hyde KD, Karunarathna SC, Deng C et al. 2018 – New species of Camptophora and Cyphellophora from China, and first report of sexual morphs for these genera. Phytotaxa 343(2), 149–159.

Zhuang JL, Zhu MQ, Zhang R, Yin H et al. 2010 – Phialophora sessilis, a species causing flyspeck signs on bamboo in China. Mycotaxon 113, 405–413.

 

Entry by

Qing Tian, Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand

 

Published online 16 December 2021